Lactate Dehydrogenase in Human Oral Neoplastic Tissues

[Lactate dehydrogenase in human oral neoplastic tissues].

要 旨:エ ナメル上皮腫,歯 肉癌 および唾液腺腫瘍 の各種 口腔領 域上皮性腫瘍組織 につ いて,DNA量 あた りのLDH活 性 とLDH isoenzyme patternを 正常対 照組織 と比較 して検討 した。 LDH活 性 は,Wroblewski and LaDueの 方 法に準 じてNADHの 吸光度変化 に よ り測 定 した。LDH isoenzyme patternは,Dietz and Lubranoの 方 法に準 じてdisc電 気泳動 によ り分離 し,Ressler氏 法の 変法 による染色液 で酵素活性 を検 出 しdensitometryに よ り求 めた。 その結果,一 般 に腫瘍組織 においてはLDH活 性の上昇が認 め られ た。 エナメル上皮腫 のLDHisoenzyme patternはLDH-3にpeakが み られ,仔 牛 エナメル器...

Lactic Dehydrogenase in Human Neoplastic Tissues.

A definite and consistent shift in the pattern of molecular forms of lactic dehydrogenase (LDH) has been found ni a large series of malignant human neoplasms as com pared with benign tumors and normal controls. This has been associated in most cases with an absolute increase in the muscle-type LDH (migrating negatively on electrophoresis). No correlation was found between the degree of these ch...

Lactate Dehydrogenase Subunits in Normal and Neoplastic Tissues of the RaP

A kinetic procedure, based on differences in affinity of lactate dehydrogenase (LDH) subunits for L(+)-lactate, was employed to measure the M and H subunit activities of this enzyme in a variety of normal and neoplastic tissues of the rat. Total LDH activity was highest in liver (both normal and regenerating) at about 150 units per gm fresh tissue and somewhat lower in heart and femoral muscle ...

Lactate dehydrogenase subunits in normal and neoplastic tissues of the rat.

A kinetic procedure, based on differences in affinity of lactate dehydrogenase (LDH) subunits for L(+)-lactate, was employed to measure the M and H subunit activities of this enzyme in a variety of normal and neoplastic tissues of the rat. Total LDH activity was highest in liver (both normal and regenerating) at about 150 units per gm fresh tissue and somewhat lower in heart and femoral muscle ...

Lactate Dehydrogenase Isoenzymes in Foetal and Neonatal Tissues.